Department of Biological and Chemical Sciences

Research interests:

Bacterial sugar transport and its regulation

The bacterial phosphoenolpyruvate:sugar phosphotransferase system (PTS)

Research in progress:

Regulation of the uptake and utilization of non-PTS sugars by the PTS: effects of mutations and growth conditions
 

Selected Publications:

Sarah L. Sutrina, Lisa Alleyne, Keisher Hoyte and Margot Blenman (2002).  Effect of replacing the general energy-coupling proteins of the PEP:sugar phosphotransferase system of Salmonella typhimurium with their fructose-inducible counterparts on utilization of the PTS sugar glucitol. Microbiol. 148:3857-3864.

Susanne Koch, Sarah L. Sutrina, Long-Fei Wu, Jonathan Reizer, Karin Schnetz, Bodo Rak and Milton H. Saier, Jr. (1996).  Identification of a site in the phosphocarrier protein, HPr, which influences its interactions with sugar permeases of the bacterial phosphotransferase system: kinetic analyses employing site-specific mutants.  J. Bacteriol. 178:1126-1133.

Sarah L. Sutrina, Prasad Reddy, Milton H. Saier, Jr. and Jonathan Reizer (1990).  The glucose permease of Bacillus subtilis is a single polypeptide chain that functions to energize the sucrose permease. J. Biol. Chem. 265:18581-18589.

Sarah L. Sutrina, A. Michael Chin, Fred Esch and Milton H. Saier, Jr. (1988). Purification and characterization of the fructose-inducible HPr-like protein, FPr, and the fructose-specific enzyme III of the phosphoenolpyruvate:sugar phosphotransferase system of Salmonella typhimurium. J. Biol. Chem. 263:5061-5069.

Sarah L. Sutrina, E. Bruce Waygood, Frank C. Grenier and Milton H. Saier, Jr. (1987).  HPr/HPr-P phosphoryl exchange reaction catalyzed by the mannitol-specific enzyme II of the bacterial phosphotransferase system.  J. Biol. Chem. 262:2636-2641.

Education:

Bachelor of Arts, University of Colorado, Major: Mathematics

Doctor of Philosophy in Biochemistry, The Johns Hopkins University
Thesis title: Purification and Properties of Two DNA-Binding Proteins of Haemophilus influenzae

Postdoctoral Fellow, The Johns Hopkins University and The John F. Kennedy Institute
Research on lipid metabolism in human cells in culture

Postdoctoral Fellow, The University of California at San Diego
Research on the bacterial PEP:sugar phosphotransferase system